Journal: Communications Biology

Article Title: EP4-induced mitochondrial localization and cell migration mediated by CALML6 in human oral squamous cell carcinoma

doi: 10.1038/s42003-024-06231-4

Figure Lengend Snippet: A Protein expression of EP4 in human gingival fibroblasts cells (HGnF cells) and human OSCC cells (HSC-3). B The EP4 agonist induced a significant increase intracellular Ca 2+ in HSC-3 cells but not HGnF cells. C Representative pictures and quantitative analysis of the scratch assay. These results confirmed that the EP4 agonist promoted the migration of HSC-3 cells but not HGnF cells. D Proposed mechanism of the EP4 signaling-mediated increases in the Ca 2+ concentration and cell migration in HGnF cells and OSCC cells. The schema was created using BioRender. A – C Data are representative of n = 4 independent experiments. A – C Unpaired t test; ns, not significant; ** p < 0.01, *** p < 0.001. Source data and exact p values are provided as a source data file.

Article Snippet: Human gingival fibroblasts (HGnF cells) and human oral keratinocytes were purchased from ScienCell Research Laboratories (CA, USA).

Techniques: Expressing, Wound Healing Assay, Migration, Concentration Assay

Journal: International Journal of Nanomedicine

Article Title: Solid lipid nanoparticles for thermoresponsive targeting: evidence from spectrophotometry, electrochemical, and cytotoxicity studies

doi: 10.2147/IJN.S147506

Figure Lengend Snippet: Biocompatibility and cytotoxicity of the ( A ) blank and 5-FU-loaded SLNs against HGF cells after 24 hours and ( B ) 5-FU-loaded SLNs against breast cancer cells (MDA-MB-231) after 1 hour at 37°C and 39°C (data = mean ± SD, n=3). Note : ( B ) 37 and 39 represent temperature (°C) of drug release studies. Abbreviations : 5-FU, 5-fluorouracil; SLN, solid lipid nanoparticle; HGF, human gingival fibroblast; FLOM, 5-FU-loaded lauric acid and oleic acid nanoparticles prepared with mono-surfactant system; FLOD, 5-FU-loaded lauric acid and oleic acid nanoparticles prepared with double-surfactant system; FLLD, 5-FU-loaded lauric acid and linoleic acid nanoparticles prepared with double-surfactant system; LOM, lauric acid and oleic acid nanoparticles prepared with mono-surfactant system; LOD, lauric acid and oleic acid nanoparticles prepared with double-surfactant system; LLD, lauric acid and linoleic acid nanoparticles prepared with double-surfactant system.

Article Snippet: Human gingival fibroblast (HGF; PCS-201-108) cells and human breast adenocarcinoma cells (MDA-MB-231, HTB-26) were purchased from American Type Culture Collection (ATCC) (Manassas, VA, USA).

Techniques:

Journal: Journal of Functional Biomaterials

Article Title: Cytotoxicity of Methacrylate Dental Resins to Human Gingival Fibroblasts

doi: 10.3390/jfb13020056

Figure Lengend Snippet: Cytotoxicity (MTT test) of freshly cured and pre-cured (pre-incubated) resins, time-dependent cell membrane damage (LDH release), cell cycle (PI-DNA assay), oxidative stress (DCF fluorescence), induction of apoptosis/necrosis (annexin V-FITC/PI), expression of heat shock protein 70 (HSP70; WB), miR-9 expression (RT-PCR) and quantification of oxidative stress-dependent HSP70 expression and in naïve human gingival fibroblast cells growing for 3 h (DCF), 6 h (DCF/HSP70 scatterplots) or 24 h (all other experiments) with methacrylate dental resin Charisma (C), Estelite (E), and Filtek Z550 (F).

Article Snippet: An adherent immortalized cell line of human gingival fibroblasts (ATCC ® CRL-2014HGF-1, f.) was grown in Dulbecco’s Modified Eagle’s Medium containing 10% fetal bovine serum (GIBCO Invitrogen, Grand Island, NE, USA) and 1% antibiotic at 37 °C, 5% CO 2 and 95% humidity.

Techniques: Membrane, Fluorescence, Expressing, Control, Activity Assay, Plasmid Preparation

Journal: Journal of Functional Biomaterials

Article Title: Cytotoxicity of Methacrylate Dental Resins to Human Gingival Fibroblasts

doi: 10.3390/jfb13020056

Figure Lengend Snippet: The effect of Charisma (C), Estelite (E), and Filtek (F) on the viability of human gingival fibroblasts is shown in panel ( A ). Cells were grown for 24 h with freshly cured dental resins (C, E, F) or with dental resins preincubated for 24 h (C′, E′, F′), and then applied to the cells. Cell viability was measured with MTT test and compared with 100% viable, naïve cells (n = 10). Panel ( B ) shows two typical histograms of propidium iodide-DNA fluorescence of control cells (a), cells co-cultured for 24 h with E (b), and bars reflecting cytotoxicity and cell cycle analysis. Cells were grown with dental resins for 24 h, stained with propidium iodide, and analyzed by flow cytometry. Original histograms were obtained using FACS Canto II flow cytometer, gating was set for the control cells and was applied to other experimental samples. Cell distribution (panel ( D )) was quantified using MultiCycle software as “subdiploid” (damaged cells), diploid (G0/G1peak)-pre-DNA synthesis/resting and proliferating cells (S-phase-DNA synthesis plus G2/M-post-DNA-synthesis/mitotic cells). Panel ( C ) shows time-dependent cytotoxicity of C, E, and F. Cells were co-cultured with dental resins and lactate dehydrogenase (LDH) activity in the culture media was measured at 1, 3, 6, 12, and 24 h. Total LDH activity was estimated in lysed control cells (*— p < 0.05 for comparison with the control cells; #— p < 0.05 for comparisons with the cells grown with C; ^— p < 0.05 for comparisons with thecells grown with E).

Article Snippet: An adherent immortalized cell line of human gingival fibroblasts (ATCC ® CRL-2014HGF-1, f.) was grown in Dulbecco’s Modified Eagle’s Medium containing 10% fetal bovine serum (GIBCO Invitrogen, Grand Island, NE, USA) and 1% antibiotic at 37 °C, 5% CO 2 and 95% humidity.

Techniques: Fluorescence, Control, Cell Culture, Cell Cycle Assay, Staining, Flow Cytometry, Software, DNA Synthesis, Activity Assay, Comparison

Journal: Journal of Functional Biomaterials

Article Title: Cytotoxicity of Methacrylate Dental Resins to Human Gingival Fibroblasts

doi: 10.3390/jfb13020056

Figure Lengend Snippet: Quantification of apoptosis/necrosis with annexin V/propidium iodide in human gingival fibroblasts grown with dental resin Charisma (C), Estelite (E), or Filtek (F) for 24 h. Part ( A )-control cells, part ( B )-necrotic cells (necrosis was induced by fifteen minutes hyperthermia at 50 °C), part ( C )-apoptotic cells (apoptosis was induced by overnight doxorubicin treatment; 1 µg/mL). Parts ( D – F ) are cells grown for 24 h with C, E, and F, respectively. Quadrant 1 represents the proportion of necrotic cells, quadrant 2 shows non-viable apoptotic cells stained with both propidium iodide and annexin V-FITC, quadrant 3 represents naïve cells, while quadrant 4 shows apoptotic cells.

Article Snippet: An adherent immortalized cell line of human gingival fibroblasts (ATCC ® CRL-2014HGF-1, f.) was grown in Dulbecco’s Modified Eagle’s Medium containing 10% fetal bovine serum (GIBCO Invitrogen, Grand Island, NE, USA) and 1% antibiotic at 37 °C, 5% CO 2 and 95% humidity.

Techniques: Control, Staining

Journal: Journal of Functional Biomaterials

Article Title: Cytotoxicity of Methacrylate Dental Resins to Human Gingival Fibroblasts

doi: 10.3390/jfb13020056

Figure Lengend Snippet: Panel ( A ) shows oxidative stress in human gingival fibroblasts grown for 3 h with light-cured dental resins: Charisma (C), Estelite (E), or Filtek (F). Cells were stained with dichlorofluorescein diacetate (DCFDA) and analyzed by flow cytometry. Typical histograms (overlay) of green DCF fluorescence in naïve human gingival fibroblast and in fibroblasts grown for 3 h with C are included. Panel ( B ) shows the expression of heat shock protein 70 (HSP70) quantified by Western-blot in cells grown for 24 h with C, E, or F. Panel ( C ) shows binary scatterplots of oxidative stress (green DCF fluorescence; relative units; damage) vs. HSP70 expression (red Alexa Fluor 647 fluorescence; relative units; repair) in cells grown for 6 h with C, E, or F. Moreover scatter area (A), central tendency lines (------) and their slopes (A ct ), and trend lines (.......) with vectors and their slopes (a t ) are displayed.

Article Snippet: An adherent immortalized cell line of human gingival fibroblasts (ATCC ® CRL-2014HGF-1, f.) was grown in Dulbecco’s Modified Eagle’s Medium containing 10% fetal bovine serum (GIBCO Invitrogen, Grand Island, NE, USA) and 1% antibiotic at 37 °C, 5% CO 2 and 95% humidity.

Techniques: Staining, Flow Cytometry, Fluorescence, Expressing, Western Blot